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KMID : 0894520010050010035
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Development & Reproduction
2001 Volume.5 No. 1 p.35 ~ p.38
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Survival Rates of Frozen-thawed Surf Clam , Spisula sachalinensis Larvae in Five Developmental Stages
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장영진
김영신/최윤희/이정용
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Abstract
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This study was peformed to find out the optimum larval stage among trochophore, early Dshaped larva, late D-shaped larva, early umbo and late umbo stages for cryopreservation of surf clam, Spisula sachalinensis larvae. Dimethyl sulfoxide (DMSO)and ethylene glycol were used as cryoprotectant, The larvae were immersed to cryoprotectants for 10 minutes and thereafter, cryopreserved in liquid nitrogen. Survival rates of trochophores frozen-thawed in 2.0 M dimethyl sulfoxide and 2.0 M ethylene glycol were the highest as 97.4% and 85.0%, respectively and post-thaw survival rates were decreased with the larval growth.
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KEYWORD
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